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A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes
Ozhalici-Unal, H., Lee Pow, C., Marks, S. A., Jesper, L. D., Silva, G. L., Shank, N. I., Jones, E. W., Burnette, J. M., Berget, P. B., & Armitage, B. A. (2008). A rainbow of fluoromodules: A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes. Journal of the American Chemical Society, 130(38), 12620-12622. https://doi.org/10.1021/ja805042p
Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar K-d values and enhance fluorescence more than 100-fold. One of these FAPs also exhibits considerable promiscuity, binding with high affinity to several other fluorogenic cyanine dyes with emission wavelengths covering most of the visible and near-IR regions of the spectrum, This significantly expands the number and wavelength range of scFv-based fluoromodules.
