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Nickel-resistance-based minitransposons: New tools for genetic manipulation of environmental bacteria

Taghavi, S., Delanghe, H., Lodewyckx, C., Mergeay, M., & van der Lelie, D. (2001). Nickel-resistance-based minitransposons: New tools for genetic manipulation of environmental bacteria. Applied and Environmental Microbiology, 67(2), 1015-1019.

Abstract

The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre mini-transposons in members of the alpha, beta, and gamma subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains

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