RTI uses cookies to offer you the best experience online. By clicking “accept” on this website, you opt in and you agree to the use of cookies. If you would like to know more about how RTI uses cookies and how to manage them please view our Privacy Policy here. You can “opt out” or change your mind by visiting: http://optout.aboutads.info/. Click “accept” to agree.
Long range dynamic effects of point-mutations trap a response regulator in an active conformation
Bobay, BG., Thompson, RJ., Hoch, JA., & Cavanagh, J. (2010). Long range dynamic effects of point-mutations trap a response regulator in an active conformation. FEBS Letters, 584(19), 4203-4207. https://doi.org/10.1016/j.febslet.2010.08.051
When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This 'conformational trapping' results in functionally hyperactive Spo0F. Consequently, point-mutations are seen to affect functionally critical motions both close to and far from the mutational site.