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HDAC inhibitors may regulate EMT and surfactant protein C in human alveolar epithelial cells
D'Souza, V. (2015). HDAC inhibitors may regulate EMT and surfactant protein C in human alveolar epithelial cells. European Respiratory Journal, Supplement, 46(PA939). https://doi.org/10.1183/13993003.congress-2015.PA939
The putative progenitor alveolar epithelial type II (ATII) cells restore epithelial barrier integrity by transdifferentiating into alveolar type I (ATI) cells. We investigated to see whether inhibition of histone deacetylases (HDAC) could reverse epithelial to mesenchymal (EMT) like intermediate phenotype during differentiation of ATII cells.
Methods: Primary human ATII cells in monolayer or co-cultured with fibroblasts were treated with 5µM of HDAC inhibitor (HDACi) MS-275 (MS) and 10ng/ml of TGFβ to assess molecular targets.
Results: MS upregulated the gradual downregulated expression of surfactant protein C (SPC) - marker of ATII cells - in both monolayer and co-culture (p Conclusion: These results show that HDAC inhibitors may regulate EMT-like phenotype during transdifferentiation of ATII cells and promote expression of SPC by repression of transcription factor Hop.