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Genetically encoded optical activation of DNA recombination in human cells
Luo, J., Arbely, E., Zhang, J., Chou, C., Uprety, R., Chin, J. W., & Deiters, A. (2016). Genetically encoded optical activation of DNA recombination in human cells. Chemical Communications, 52(55), 8529-8532. https://doi.org/10.1039/c6cc03934k
We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.