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ENU mutagenesis in the mouse electrophoretic specific-locus test. 2. Mutational studies of mature oocytes
Lewis, S., Barnett, L., & Shelby, M. D. (1992). ENU mutagenesis in the mouse electrophoretic specific-locus test. 2. Mutational studies of mature oocytes. Mutation Research - Reviews in Genetic Toxicology, 296(1-2), 129-133. https://doi.org/10.1016/0165-1110(92)90036-9
Experiments were conducted using the biochemical specific-locus test to assess the mutagenicity of N-ethyl-N-nitrosourea (ENU) in mature oocytes of mice. C57Bl/6J females were treated with 100 mg/kg ENU by intraperitoneal injection and mated to untreated DBA males for 1 week following treatment. 1447 progeny were screened for evidence of mutations affecting the electrophoretic mobility of 32 different proteins; two mutants were detected by electrophoretic analyses. These results provide evidence that ENU is a germ-cell mutagen in mouse mature oocytes, although the frequency of mutants is somewhat lower than that obtained from spermatogonia treated with the same dose