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Development of an analytical method for quantitation of deoxynivalenol by UPLC-MS-MS
A preliminary assessment of gestational and lactational transfer in rats
Rehder Silinski, M. A., Gilliam, J. A., Fernando, R. A., Robinson, V. G., Germolec, D., Cunny, H., Huang, M. C., Furr, J., & Waidyanatha, S. (2021). Development of an analytical method for quantitation of deoxynivalenol by UPLC-MS-MS: A preliminary assessment of gestational and lactational transfer in rats. Journal of Analytical Toxicology, 45(6), 566-572. https://doi.org/10.1093/jat/bkaa119
Deoxynivalenol (DON) is the most widely distributed trichothecene mycotoxin in grain-based foods and animal feed. Exposure to DON is widespread as it has been detected in food sources from around the world. The objective of this work was to develop a method to quantitate DON in biological matrices and apply it in a preliminary assessment of gestational and lactational transfer of DON following exposure of pregnant rats. The method used protein precipitation followed by ultra-performance liquid chromatography-tandem mass spectrometry. The method was evaluated in male Sprague Dawley rat plasma over the concentration range similar to 2-1,000 ng/mL. The method was linear (r >= 0.99), accurate (mean relative error <= +/- 4.9%) and precise (relative standard deviation <= 5.5%). The mean absolute recovery was 85.9%. The limit of detection was 0.35 ng/mL. The method was also evaluated in gestational day (GD) 18 Hsd:Sprague Dawley (R) SD (R) dam plasma and fetal homogenate (mean % relative error <= +/- 16.9; % relative standard deviation <= 9.5). Concentrations of DON in dam plasma stored at -80 degrees C for at least 29 days and in fetal homogenate for at least 43 days were within 97.9 to 120% of Day 0 concentrations, demonstrating that DON is stable in these matrices. The method was used to quantitate DON in rat maternal plasma, amniotic fluid, GD 18 fetuses and postnatal day (PND) 4 pups following exposure of dams to 0 (control) and 1 mg/kg DON beginning on GD 6 and continuing through gestation and lactation for a preliminary assessment of maternal transfer. In animals exposed to 1 mg/kg/day, similar concentration of DON was found in GD 18 dam plasma and fetuses, demonstrating significant gestational transfer. The concentration of DON in PND 4 dam plasma was similar to that in GD 18 dam plasma. However, DON was not detected in PND 4 pup plasma above the limit of detection of the assay, demonstrating absence of transfer of DON to pups via lactation.
