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The authors investigated possibilities for nanotechnological eco-innovation in the Danish polymer industry. Through interviews with employees of two companies and stakeholders engaged in regulation and research and development, the authors found drivers of eco-innovation to be material applications, public funding and market opportunities. They also identified barriers to eco-innovation including the lack of industrial and regulatory competencies and standards as well as limited coordination between agencies and councils responsible for allocating R&D funds. Furthermore, capability building and coordination of efforts among stakeholders are needed for the successful development of eco-innovation using nanomaterials. The authors suggest using the dynamics between regulation, R&D, and industry to promote eco-innovation.