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Dawkins, J. O., Vidanapathirana, A. K., Thompson, L. C., Sumner, S. C. J., Fennell, T. R., Brown, J. M., & Wingard, C. J. (2013). Silver nanoparticle exposure increases vasoconstrictor response in nonpregnant female Sprague-Dawley rats. The Toxicologist, Supplement to Toxicological Sciences, 132(1), 242.
The use and production of silver nanoparticles (AgNP) is growing rapidly raising concerns regarding their safety to human health, particularly following translocation to the circulatory system. Previous findings from our lab have shown intravenous (IV) exposure to AgNP changes the vasoconstrictor response in both pregnant and male Sprague Dawley (SD) rats. We hypothesized, acute IV exposure to AgNP in non-pregnant females will increase vascular reactivity of arterial vessels SOT 2013 ANNUAL MEETING 243 from mesenteric and uterine vascular beds and aortas and these changes will be influenced by NP size and coating. Female, non-pregnant, SD rats were intravenously exposed to 200 μg of 20 or 110 nm AgNP coated with polyvinylpyrorrolidone (PVP) or citrate and suspended in water. 24 hrs. post-exposure, wire myography tested the vasomotor responses in aortic, first order mesenteric and main uterine artery vessel segments. Cumulative dose response curves were created for phenylephrine (PE), angiotensin II (ANGII), and endothelin 1 (ET1). Segments of uterine artery and aorta from AgNP exposed animals generated larger stress when compared to vehicle controls. No significant differences were observed in the mesenteric artery responses. Maximum stress values in the uterine artery were greater (p <0.05) in response to ET1 and ANGII stimulation following exposure to 110 nm PVP AgNP (16.4 ± 2.7 and 14.5 ± 2.2 mN/mm2, respectively) as compared to 110 nm citrate AgNP (8.0 ± 1.0 and 6.9 ± 0.8 mN/mm2, respectively). Conversely, maximum stress in response to PE in aortic segments was greater (p < 0.01) following exposure to 20 nm PVP AgNP (1.3 ± 0.2 mN/mm2) as compared to 20 citrate nm AgNP (2.8 ± 0.3 mN/mm2). Our results suggest IV exposure to AgNP has differential effects on increasing the vasoconstrictor responses that are dependent on the vascular bed, size of the NP and type of coating. This work is supported by NIEHS U19 ES019525.
