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Preliminary Molecular Analysis of the TK Locus in L5178Y Large- and Small-Colony Mouse Lymphoma Cell Mutants
Cobb, R., Martin, J., Korytynski, E., Monteith, L., & Hughes, T. J. (1989). Preliminary Molecular Analysis of the TK Locus in L5178Y Large- and Small-Colony Mouse Lymphoma Cell Mutants. Mutation Research Letters, 226(4), 253-258. https://doi.org/10.1016/0165-7992(89)90078-X
Mouse lymphoma cells of the L5178Y TK+/- -3.7.2C line were exposed to sidestream and mainstream cigarette smoke condensates (CSC). Cells which survived the trifluorothymidine (TFT) challenge fell in 2 classes: large- and small-colony formers. Southern blot analysis of NcoI-digested DNA from mutant colonies yielded 2 distinct restriction fragment banding patterns when probed with the thymidine kinase (TK) cDNA clone pMtk4. One such pattern was composed of 4 bands at 6.4, 5.5, 4.7 and 2.9 kilobase pairs (kb) and was identical to that of TK+/- controls. A second pattern differed from the first only in the absence of the 6.4-kb band. The majority (83/95) of both large and small colonies derived from cells exposed to CSC exhibited restriction fragment banding patterns lacking the 6.4-kb band. The data from the present study suggest that there is no association between mutant colony size and the presence of the 6.4-kb NcoI restriction fragment at the TK locus in the mouse lymphoma mutants analyzed.