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Mouse Spermatogonia Exposed to a High, Multiply Fractionated Dose of a Cancer Chemotherapeutic Drug: Mutation Analysis by Electrophoresis
Johnson, F. M., & Lewis, S. (1981). Mouse Spermatogonia Exposed to a High, Multiply Fractionated Dose of a Cancer Chemotherapeutic Drug: Mutation Analysis by Electrophoresis. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 81(2), 197-202. https://doi.org/10.1016/0027-5107(81)90034-8
Male mice of the DBA/2J strain were injected with procarbazine at a dose of 200 mg/kg body weight twice weekly until an accumulated dose of 2400 mg/kg was reached. A concurrent control group, injected only with the vehicle (saline) was also established. Most of the treated animals died as a result of exposure and all survivors became temporarily sterile. After regaining fertility the few survivors were repeatedly mated with C57BL/6J females over several weeks time to generate a population of F1 animals. The parental animals and the F1 were subsequently analyzed by electrophoresis for the occurrence of newly arisen mutations of spermatogonial origin. A mutation in the gene Pep-3 was found.