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Genetic mapping and characterization of Pseudomonas aeruginosa mutants that hyperproduce exoproteins

Bjorklind, A., Wretlind, B., Mollegard, I., Schad, P., Iglewski, BH., & Cox, CD. (1985). Genetic mapping and characterization of Pseudomonas aeruginosa mutants that hyperproduce exoproteins. Journal of Bacteriology, 162(3), 1329-1331. http://jb.asm.org/cgi/content/abstract/162/3/1329

Abstract

We isolated two mutants of Pseudomonas aeruginosa PAO with defective iron uptake. In contrast to the wild-type strain, the mutants produced extracellular protease activity in media containing high concentrations of salts or iron and hyperproduced elastase, staphylolytic enzyme, and exotoxin A in ordinary media (Xch mutants). The mutations were located in the 55' region of the chromosome, between the markers met-9011 and pyrD.

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