RTI uses cookies to offer you the best experience online. By clicking “accept” on this website, you opt in and you agree to the use of cookies. If you would like to know more about how RTI uses cookies and how to manage them please view our Privacy Policy here. You can “opt out” or change your mind by visiting: http://optout.aboutads.info/. Click “accept” to agree.
Dopamine transporter synthesis and degradation rate in rat striatum and nucleus accumbens using RTI-76
Kimmel, HL., Carroll, F., & Kuhar, MJ. (2000). Dopamine transporter synthesis and degradation rate in rat striatum and nucleus accumbens using RTI-76. Neuropharmacology, 39(4), 578-585.
Intracerebroventricular injections of the irreversible dopamine transporter (DAT) inhibitor, RTI-76 (3 beta-(3-p-chlorophenyl) tropan-2 beta-carboxylic acid p-isothiocyanatophenylethyl ester hydrochloride), decreased DAT binding in both the striatum and nucleus accumbens as measured by both [H-3]GBR12935 and by [H-3]WIN35,428. This decrease was dose-related, with 100 nmol RTI-76 producing approximately a 50% decrease in both regions. The maximal inhibition of DAT binding was observed 24 h after RTI-76 injection, and binding was fully restored 7 days after injection. The DAT protein half-life determined under these conditions was about 2 days. [H-3]Nisoxetine binding at norepinephrine transporters in the cortex was not altered by RTI-76 administration at any time point or dose examined. (C) 2000 Elsevier Science Ltd. All rights reserved